Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2976Y] to TdT
Suitable for: Flow Cyt, WB, IP, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-TdT antibody [EPR2976Y]
See all TdT primary antibodies
Description

Rabbit monoclonal [EPR2976Y] to TdT
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human TdT (C terminal).
Positive control
- Human thymus and Jurkat lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2976Y
Isotype

IgG
Research areas

Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)

Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution (purified) + Human fetal thymus lysates at 15 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 58 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (ab76544)

ab76544 (purified) at 1:20 dilution (2μg) immunoprecipitating TdT in Jurkat (human acute T cell leukemia) whole cell lysate.

Lane 1 (input): Jurkat (human acute T cell leukemia) whole cell lysate 10μg
Lane 2 (+): ab76544 & Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76544 in Jurkat (human acute T cell leukemia) whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-TdT antibody [EPR2976Y] (ab76544)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TdT with purified ab76544 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] (ab76544)

Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling TdT with Purified ab76544 at 1:100 dilution (1.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)

All lanes : Anti-TdT antibody [EPR2976Y] (ab76544) at 1/2000 dilution (purified)

Lane 1 : Jurkat (human acute T cell leukemia) whole cell lysates
Lane 2 : Mouse thymus lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 58 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)

Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution (purified) + Rat thymus lysates at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 58 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)

All lanes : Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution (unpurified)

Lane 1 : Human thymus lysate
Lane 2 : Jurkat lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 24 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Anti-TdT antibody [EPR2976Y] (ab76544)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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