Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2976Y] to TdT - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TdT antibody [EPR2976Y] - BSA and Azide free
    See all TdT primary antibodies

  • Description

    Rabbit monoclonal [EPR2976Y] to TdT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MOLT-4 cells.

  • General notes

    Ab232378 is the carrier-free version of ab76544. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR2976Y

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)
    Immunoprecipitation - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)

    ab76544 (purified) at 1:20 dilution (2μg) immunoprecipitating TdT in Jurkat (human acute T cell leukemia) whole cell lysate.

    Lane 1 (input): Jurkat (human acute T cell leukemia) whole cell lysate 10μg
    Lane 2 (+): ab76544 & Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76544 in Jurkat (human acute T cell leukemia) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

  • Flow Cytometry - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)
    Flow Cytometry - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TdT with purified ab76544 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

  • Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)
    Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)

    Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling TdT with Purified ab76544 at 1:100 dilution (1.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

  • Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)
    Anti-TdT antibody [EPR2976Y] - BSA and Azide free (ab232378)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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