Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22857-38] to COMP/Cartilage oligomeric matrix protein
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38]
    See all COMP/Cartilage oligomeric matrix protein primary antibodies

  • Description

    Rabbit monoclonal [EPR22857-38] to COMP/Cartilage oligomeric matrix protein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, rat and mouse articular cartilage lysate. IP: Human and mouse articular cartilage lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)
    Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)
    All lanes : Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037) at 1/5000 dilution

    Lane 1 : Human articular cartilage lysate
    Lane 2 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate
    Lane 3 : Mouse articular cartilage lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 82 kDa
    Observed band size: 100,250 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9143347, 24917676). 

    Negative control: Jurkat (Database: EBI Expression Atlas).

    Exposure time: Lanes 1-2: 1 second; Lane 3: 125 seconds.

  • Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)
    Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)
    Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037) at 1/5000 dilution + Rat articular cartilage lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 82 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9143347, 24917676).

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)
    Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)

    COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg human articular cartilage lysate 10ug with ab260037 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260037 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human articular cartilage lysate 10ug

    Lane 2: ab260037 IP in human articular cartilage lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260037 in human articular cartilage lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)
    Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)

    COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg mouse articular cartilage lysate 10ug with ab260037 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260037 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse articular cartilage lysate 10ug

    Lane 2: ab260037 IP in mouse articular cartilage lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260037 in mouse articular cartilage lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)
    Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR22857-38] (ab260037)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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