Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358)
Key features and details
- Expression system: HEK 293 cells
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, WB
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Product name
Recombinant Human COMP/Cartilage oligomeric matrix protein
See all COMP/Cartilage oligomeric matrix protein proteins and peptides -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
HVDYKDDDDKPAGQGQSPLGSDLGPQMLRELQETNAALQDVRE LLRQQ VREITFLKNTVMECDACGMQQSVRTGLPSVRPLLHCAPGFCFPGV ACI QTESGARCGPCPAGFTGNGSHCTDVNECNAHPCFPRVRCINTSPGFR C EACPPGYSGPTHQGVGLAFAKANKQVCTDINECETGQHNCVPNSVCINT RGSFQCGPCQPGFVGDQASGCQRRAQRFCPDGSPSECHEHADCVLERDG S RSCVCAVGWAGNGILCGRDTDLDGFPDEKLRCPERQCRKDNCVTVPN SGQ EDVDRDGIGDACDPDADGDGVPNEKDNCPLVRNPDQRNTDEDKWG DACDN CRSQKNDDQKDTDQDGRGDACDDDIDGDRIRNQADNCPRVPNS DQKDSDG DGIGDACDNCPQKSNPDQADVDHDFVGDACDSDQDQDGDGH QDSRDNCPT VPNSAQEDSDHDGQGDACDDDDDNDGVPDSRDNCRLVPN PGQEDADRDGV GDVCQDDFDADKVVDKIDVCPENAEVTLTDFRAFQTV VLDPEGDAQIDPN WVVLNQGREIVQTMNSDPGLAVGYTAFNGVDFEGT FHVNTVTDDDYAGFI FGYQDSSSFYVVMWKQMEQTYWQANPFRAVAEP GIQLKAVKSSTGPGEQL RNALWHTGDTESQVRLLWKDPRNVGWKDKKS YRWFLQHRPQVGYIRVRFY EGPELVADSNVVLDTTMRGGRLGVFCFSQ ENIIWANLRYRCNDTIPEDYE THQLRQA -
Predicted molecular weight
82 kDa including tags -
Amino acids
21 to 757 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
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Stability and Storage
Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
Constituents: 0.242% Tris, 0.29% Sodium chloride
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ReconstitutionAdd deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.
Images
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SDS-PAGE - Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358)14% SDS-PAGE showing ab104358 at approximately 82.4kDa:
Lane 1: M.W. marker – 21, 31, 45, 66, 97 kDa.
Lane 2: reduced and boiled sample, 5μg/lane.
Lane 3: non-reduced and non-boiled sample, 5μg/lane. -
Western blot - Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358)Anti-COMP/Cartilage oligomeric matrix protein antibody (ab42225) at 1/1000 dilution +Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 minutes
