Human COMP ELISA Kit (ab275907)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 116 pg/ml
- Range: 781.25 pg/ml - 50000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human COMP ELISA Kit
See all COMP/Cartilage oligomeric matrix protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 4.5% Inter-assay Sample n Mean SD CV% Plasma 3 5.4% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
116 pg/ml -
Range
781.25 pg/ml - 50000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 94% - 115% Serum 104 102% - 105% EDTA Plasma 117 114% - 118% Cit plasma 115 113% - 116% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human COMP ELISA kit (ab275907) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human COMP protein in human serum, plasma, and cell culture supernatants samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human COMP with 116 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
COMP, Cartilage oligomeric matrix protein also known as Thrombospondin-5, is an extracellular matrix protein involved in the stabilization of cartilage. It also serves as a potent cell signaling mediator through its interactions with Caspase-3 and IAP family proteins. Antibodies for this kit were raised against a recombinant C-terminal fragment of this protein. This protein is homologous in mouse and rat, sharing over 90% sequence identity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human COMP Capture Antibody 1 x 600ml 10X Human COMP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Human COMP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. -
Tissue specificity
Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect. -
Involvement in disease
Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 8 TSP type-3 repeats. -
Developmental stage
Present during the earliest stages of limb maturation and is later found in regions where the joints develop. -
Domain
The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
The TSP C-terminal domain mediates interaction with FN1 and ACAN. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
Alternative names
- Cartilage oligomeric matrix protein
- cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple)
- Cartilage oligomeric matrix protein precursor
see all -
Database links
- Entrez Gene: 1311 Human
- Omim: 600310 Human
- SwissProt: P49747 Human
- Unigene: 1584 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human COMP standard curve in 1X Cell Extraction Buffer PTR.Example of human COMP standard curve in 1X Cell Extraction Buffer PTR.
-
Interpolated concentrations of native COMP in human serum, plasma, and cell culture supernatant samples.Interpolated concentrations of native COMP in human serum, plasma, and cell culture supernatant samples.
-
Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).The mean COMP concentration was determined to be 1.7 µg/mL with a range of 0.7 – 3 µg/mL.
-
Sandwich ELISA - Human COMP ELISA Kit (ab275907)To learn more about the advantages of recombinant antibodies see here.
