Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19780] to CXCL7/PBP - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free
    See all CXCL7/PBP primary antibodies

  • Description

    Rabbit monoclonal [EPR19780] to CXCL7/PBP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251462 is the carrier-free version of ab206966. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251462 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)
    Western blot - Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)
    Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution + Human NAP2 active recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 11 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab206966, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)
    Western blot - Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)
    All lanes : Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution

    Lane 1 : Human serum at 20 µg
    Lane 2 : Human spleen lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 11 kDa why is the actual band size different from the predicted?



    This data was developed using ab206966, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes.

  • Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)
    Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)
    This data was developed using ab206966, the same antibody clone in a different buffer formulation.NAP2 was immunoprecipitated from 0.35 mg of Human serum with ab206966 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206966 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: Human serum 10µg (Input). Lane 2: ab206966 IP in Human serum. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206966 in Human serum. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 5 seconds. The band at 50 kD is likely to be human IgG in input and rabbit IgG heavy chain in +ve and –ve lane, but this has not been tested.
  • Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)
    Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (ab251462)

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