Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Uteroglobin antibody [EPR19846] (ab213203)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19846] to Uteroglobin
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat

Product name

Anti-Uteroglobin antibody [EPR19846]
See all Uteroglobin primary antibodies
Description

Rabbit monoclonal [EPR19846] to Uteroglobin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lung lysate. IHC-P: Mouse and rat lung tissues. IHC-Fr: Mouse and rat lung tissues. IP: Mouse lung lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19846
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on the airway epithelium of rat lung is observed [PMID: 22916196].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, paraffin-embedded mouse lung tissue labeling Uteroglobin antibody with ab213203 at 1/1000 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution.

Positive staining of Uteroglobin on the airway epithelium of mouse lung is observed.

The nuclear counterstain is DAPI (blue).

Heat mediated antigen retrieval using citrate buffer pH 6.0

The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
Western blot - Anti-Uteroglobin antibody [EPR19846] (ab213203)

All lanes : Anti-Uteroglobin antibody [EPR19846] (ab213203) at 1/1000 dilution

Lane 1 : Mouse lung lysate at 20 µg
Lane 2 : Mouse heart lysate at 30 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 10 kDa
Observed band size: 10 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on the airway epithelium of mouse lung. However, there is some non-specific cytoplasmic staining on the alveolar cells of mouse lung. The correct staining on the airway epithelium of mouse lung is observed when using immunofluorescence detection method.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Uteroglobin with ab213203 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Positive staining on the airway epithelium of mouse lung [PMID: 22916196].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat lung tissue labeling Uteroglobin with ab213203 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Positive staining on the airway epithelium of rat lung [PMID: 22916196].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (ab213203)

Uteroglobin was immunoprecipitated from 0.35 mg of mouse lung lysate with ab213203 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab213203 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: Mouse lung lysate 10ug (Input).

Lane 2: ab213203 IP in mouse lung lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213203 in mouse lung lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-Uteroglobin antibody [EPR19846] (ab213203)

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