Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Uteroglobin antibody [EPR19846] (ab213203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19846] to Uteroglobin
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Uteroglobin antibody [EPR19846]
    See all Uteroglobin primary antibodies

  • Description

    Rabbit monoclonal [EPR19846] to Uteroglobin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung lysate. IHC-P: Mouse and rat lung tissues. IHC-Fr: Mouse and rat lung tissues. IP: Mouse lung lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19846

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Strong cytoplasmic staining on the airway epithelium of rat lung is observed [PMID: 22916196].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, paraffin-embedded mouse lung tissue labeling Uteroglobin antibody with ab213203 at 1/1000 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution.

    Positive staining of Uteroglobin on the airway epithelium of mouse lung is observed.

    The nuclear counterstain is DAPI (blue).

    Heat mediated antigen retrieval using citrate buffer pH 6.0

    The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

  • Western blot - Anti-Uteroglobin antibody [EPR19846] (ab213203)
    Western blot - Anti-Uteroglobin antibody [EPR19846] (ab213203)
    All lanes : Anti-Uteroglobin antibody [EPR19846] (ab213203) at 1/1000 dilution

    Lane 1 : Mouse lung lysate at 20 µg
    Lane 2 : Mouse heart lysate at 30 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on the airway epithelium of mouse lung. However, there is some non-specific cytoplasmic staining on the alveolar cells of mouse lung. The correct staining on the airway epithelium of mouse lung is observed when using immunofluorescence detection method.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
    Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Uteroglobin with ab213203 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Positive staining on the airway epithelium of mouse lung [PMID: 22916196].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)
    Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (ab213203)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat lung tissue labeling Uteroglobin with ab213203 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Positive staining on the airway epithelium of rat lung [PMID: 22916196].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (ab213203)
    Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (ab213203)

    Uteroglobin was immunoprecipitated from 0.35 mg of mouse lung lysate with ab213203 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213203 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse lung lysate 10ug (Input).

    Lane 2: ab213203 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213203 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-Uteroglobin antibody [EPR19846] (ab213203)
    Anti-Uteroglobin antibody [EPR19846] (ab213203)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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