Human Uteroglobin ELISA Kit (ab238266)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 pg/ml
- Range: 6.3 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Uteroglobin ELISA Kit
See all Uteroglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.3% Inter-assay Sample n Mean SD CV% Serum 3 4.6% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.4 pg/ml -
Range
6.3 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 95% - 102% Saliva 101 94% - 110% Milk 107 98% - 124% Urine 106 94% - 119% Serum 97 90% - 105% Hep Plasma 101 95% - 109% EDTA Plasma 100 88% - 111% Cit plasma 89 82% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Uteroglobin ELISA Kit (ab238266) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Uteroglobin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Uteroglobin with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Uteroglobin Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Uteroglobin Detector Antibody (Lyophilized) 1 vial Human Uteroglobin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2. -
Tissue specificity
Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways). -
Sequence similarities
Belongs to the secretoglobin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Blastokinin
- CC10
- CC16
see all -
Database links
- Entrez Gene: 7356 Human
- Omim: 192020 Human
- SwissProt: P11684 Human
- Unigene: 523732 Human
Images
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Other - Human Uteroglobin ELISA Kit (ab238266)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Uteroglobin Standard Curve Comparison.Standard Curve comparison between Human Uteroglobin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of human Uteroglobin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Uteroglobin in human serum and plasma samples.The concentrations of Uteroglobin were measured in duplicates, interpolated from the Uteroglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 100, plasma (citrate) 1: 100, plasma (heparin) 1: 100, and plasma (EDTA) 1: 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin concentration was determined to be 13.2 ng/mL in serum, 14.5 ng/mL in plasma (citrate), 10.7 ng/ml in plasma (heparin), and 11.4 ng/ml in plasma (EDTA). -
Interpolated concentrations of native Uteroglobin in female human urine, saliva, and milk samples.The concentrations of Uteroglobin were measured in duplicates, interpolated from the Uteroglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine (2.5%), saliva (1: 2000), and milk (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin concentration was determined to be 5.4 ng/mL in urine, 279.8 ng/mL in saliva, and 419.2 pg/mL in milk. -
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin protein concentration was determined to be 14.7 ng/mL with a range of 6.0 - 30.0 ng/mL.
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Sandwich ELISA - Human Uteroglobin ELISA Kit (ab238266)To learn more about the advantages of recombinant antibodies see here.
