Anti-USP25 antibody [EPR15019] (ab187156)
![Anti-USP25 antibody [EPR15019] (ab187156)](https://www.abcam.com/ps/products/187/ab187156/Images/ab187156-220326-anti-usp25-antibody-epr15019-western-blot.jpg "Anti-USP25 antibody [EPR15019] (ab187156)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15019] to USP25
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-USP25 antibody [EPR15019]
See all USP25 primary antibodies -
Description
Rabbit monoclonal [EPR15019] to USP25 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, A673, Raw264.7, PC-12 and BxPC-3 cell lysate. A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15019 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-USP25 antibody [EPR15019] (ab187156)All lanes : Anti-USP25 antibody [EPR15019] (ab187156) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : A673 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 122 kDa
Observed band size: 122 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-USP25 antibody [EPR15019] (ab187156)](https://www.abcam.com/ps/products/187/ab187156/Images/ab187156-220324-anti-usp25-antibody-epr15019-western-blot.jpg)
Western blot - Anti-USP25 antibody [EPR15019] (ab187156)All lanes : Anti-USP25 antibody [EPR15019] (ab187156) at 1/1000 dilution
Lane 1 : Raw264.7 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 122 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Flow Cytometry - Anti-USP25 antibody [EPR15019] (ab187156)](https://www.abcam.com/ps/products/187/ab187156/Images/ab187156-220323-anti-usp25-antibody-epr15019-flow-cytometry.jpg)
Flow Cytometry - Anti-USP25 antibody [EPR15019] (ab187156)Flow cytometry analysis of USP25 expression in 2% paraformaldehyde-fixed A673 cells using ab187156 at 1/60 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody.
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![Anti-USP25 antibody [EPR15019] (ab187156)](https://www.abcam.com/ps/products/187/ab187156/Images/ab187156-4-benefits-of-recombinant-antibodies.png)
Anti-USP25 antibody [EPR15019] (ab187156)
