Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸ 10 µl 113 913 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPRR17575] to Claudin 4
Suitable for: IHC-P, WB, IP
Reacts with: Human

Product name

Anti-Claudin 4 antibody [EPRR17575]
See all Claudin 4 primary antibodies
Description

Rabbit monoclonal [EPRR17575] to Claudin 4
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: LNCaP, PC-3, SW480, MCF7, SK-OV-3 and NIH:OVCAR-3 whole cell lysates. IHC-P: Human colon, endometrium, liver, cholangiocarcinoma and breast cancer tissues. IP: SW480 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPRR17575
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Claudin 4 with ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane and staining on epithelial cells of human colon is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Lanes 1-2 : Anti-Claudin 4 antibody [EPRR17575] (ab210796) at 1/2000 dilution
Lanes 3-6 : Anti-Claudin 4 antibody [EPRR17575] (ab210796) at 1/1000 dilution

Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
Lane 6 : NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 22 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/2/3/6: 1 minute; Lane 4: 30 seconds; Lane 5: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Claudin 4 with ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on epithelial cells of human endometrium is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Claudin 4 with ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Weak membrane staining on biliary epithelium of human liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue labeling Claudin 4 with ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on human cholangiocarcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Claudin 4 with ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative staining on human hepatocellular carcinoma.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Claudin 4 with ab210796 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on epithelial cells of human breast cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Claudin 4 antibody [EPRR17575] (ab210796)

Claudin 4 was immunoprecipitated from 1mg of SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate with ab210796 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab210796 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: SW480 whole cell lysate 10µg (Input).

Lane 2: ab210796 IP in SW480 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210796 in SW480 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Claudin 4 antibody [EPRR17575] (ab210796)

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