Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
![Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)](https://www.abcam.com/ps/products/7/ab7856/Images/ab7856-448954-antihladrdpdqimmunohistochemistrytonsilhuman.jpg "Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)")
Key features and details
- Mouse monoclonal [CR3/43] to HLA DR + DP + DQ
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HLA DR + DP + DQ antibody [CR3/43]
See all HLA DR + DP + DQ primary antibodies -
Description
Mouse monoclonal [CR3/43] to HLA DR + DP + DQ -
Host species
Mouse -
Specificity
The gene of the human histocompatibility complex class II consists of at least four subregions: HLA DP, DQ, and DR, containing a minimum of one alpha and one beta chain. This antibody reacts with the beta-chain of all products of the gene subregions DP, DQ, and DR.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Tissue, cells or virus corresponding to Human HLA DR + DP + DQ. Raised against tonsil cells of human origin
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Positive control
- IHC-P: Human tonsil tissue
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General notes
This antibody principally labels B cells, interdigitating reticulum cells, Langerhans’ cells and manymacrophages.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
This antibody principally labels B cells, interdigitating reticulum cells, Langerhans’ cells and manymacrophages. -
Clonality
Monoclonal -
Clone number
CR3/43 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
IHC image of -HLA DR + DP + DQ staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab7856, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)](https://www.abcam.com/ps/products/7/ab7856/Images/ab7856-13310-anti-hla-dr-dp-dq-antibody-cr3-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)IHC image of ab7856 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7856, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)](https://www.abcam.com/ps/products/7/ab7856/Images/ab7856-13311-anti-hla-dr-dp-dq-antibody-cr3-43-western-blot.jpg)
Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)All lanes : Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) at 1/500 dilution
Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 27 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)](https://www.abcam.com/ps/products/7/ab7856/Images/ab7856-305369-anti-hla-dr-dp-dq-antibody-cr3-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) This image is courtesy of an anonymous AbreviewAb7856 staining HLA DR+DP+DQ in Human Colorectal tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation at pH6 for 20minutes. Samples were fixed with formaldehyde and blocked with 10% hydrogen peroxide for 5 minutes. Samples were incubated with primary antibody at 1/100 dilution for 15 minutes. HRP rabbit polyclonal polymer refine detection kit was used as the secondary antibody.