Anti-Urocortin 3 antibody (ab79121)
Key features and details
- Rabbit polyclonal to Urocortin 3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Urocortin 3 antibody
See all Urocortin 3 primary antibodies -
Description
Rabbit polyclonal to Urocortin 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Urocortin 3 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90638) -
Positive control
- This antibody gave a positive signal in human brain pituitary tissue lysate. IF/ICC: HepG2 cell line.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Relevance
Urocortin 3 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. A powerful cytoprotective effect of urocortin peptide administration against ischemia and reperfusion injury has been demonstrated in isolated cardiomyocyte models, as well as in the intact heart both in vitro and in vivo. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 114131 Human
- Omim: 605901 Human
- SwissProt: Q969E3 Human
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Alternative names
- MGC119002 antibody
- OTTHUMP00000018999 antibody
- Prepro urocortin 3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Urocortin 3 antibody (ab79121)
ICC/IF image of ab79121 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79121, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Urocortin 3 antibody (ab79121)Anti-Urocortin 3 antibody (ab79121) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Western blot - Anti-Urocortin 3 antibody (ab79121)
Protocols
Datasheets and documents
References (1)
ab79121 has been referenced in 1 publication.
- Kavalakatt S et al. Urocortin 3 Levels Are Impaired in Overweight Humans With and Without Type 2 Diabetes and Modulated by Exercise. Front Endocrinol (Lausanne) 10:762 (2019). PubMed: 31781037
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Urocortin 3 antibody (ab79121)
ICC/IF image of ab79121 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79121, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Urocortin 3 antibody (ab79121)Anti-Urocortin 3 antibody (ab79121) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Western blot - Anti-Urocortin 3 antibody (ab79121)
