Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)
![Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)](https://www.abcam.com/ps/products/271/ab271290/Images/ab271290-395364-anticd271-antibody-nori146c-western-blot-neuro2a.jpg "Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [NORI146C] to p75 NGF Receptor
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse
Overview
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Product name
Anti-p75 NGF Receptor antibody [NORI146C]
See all p75 NGF Receptor primary antibodies -
Description
Rat monoclonal [NORI146C] to p75 NGF Receptor -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Neuro-2a whole cell lysate. IHC-P: Mouse spleen and liver tissue. IHC-Fr: Mouse spleen tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
NORI146C -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)Anti-p75 NGF Receptor antibody [NORI146C] (ab271290) at 0.755 µg/ml + Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
Secondary
Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 45 kDa
Exposure time: 59 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)](https://www.abcam.com/ps/products/271/ab271290/Images/ab271290-1-anticd271-antibody-nori146c-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD271 with ab271290 at 1/1000 dilution (0.755µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse spleen. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)](https://www.abcam.com/ps/products/271/ab271290/Images/ab271290-2-anticd271-antibody-nori146c-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD271 with ab271290 at 1/1000 dilution (0.755µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the peri-sinusoidal space in the mouse liver. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Frozen sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)](https://www.abcam.com/ps/products/271/ab271290/Images/ab271290-3-anticd271-antibody-nori146c-immunohistochemistry-frozen-mouse-spleen.jpg)
Immunohistochemistry (Frozen sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD271 with ab271290 at 1/100 dilution (7.55µg/ml) followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)](https://www.abcam.com/ps/products/271/ab271290/Images/ab271290-5-benefits-of-recombinant-antibodies.png)
Anti-p75 NGF Receptor antibody [NORI146C] (ab271290)
