FirePlex Human Key Cytokines - Immunoassay Panel (ab243549)
Overview
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Product name
FirePlex Human Key Cytokines - Immunoassay Panel -
Detection method
Flow cytometry-fluorescent -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Cell culture extracts, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate, Cerebral Spinal Fluid -
Assay type
Multiplex -
Assay time
3h 30m -
Species reactivity
Reacts with: Human -
Product overview
FirePlex Human Key Cytokines - Immunoassay Panel (ab243549) contains all the reagents you need to run a 17-plex assay: antibody conjugated particles, Human Core Reagents and Protein Standard Mix.
NOTE: FirePlex Immunoassay panels require the purchase of a Run Buffer. Use our FirePlex Cytometer Setup Kit V2 (ab245835) to select the most appropriate Run Buffer for your flow cytometer.
Assay run-time is 3.5 hours, followed by particle analysis using a validated flow cytometer model and data analysis using our integrated, free-of-charge FirePlex Analysis Workbench software.
FirePlex® is a registered trade mark in the United States and is registered as a European Union Trade Mark.
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Notes
This panel was previously available under the product code ab231392/ab229791, which is discontinued. We have redeveloped this panel using new, recombinant monoclonal antibodies that provide higher sensitivity and better reproducibility when quantifying the analytes with biofluid samples, improving consistency in kit performance.
The following information provides the sensitivity, range, and required Protein Standard Mix.Target
Sensitivity
(pg/mL)Dynamic Range
(pg/mL)Protein
StandardGM-CSF
0.62
4.57 - 10,000
Mix A
IFN gamma
3.49
4.57 - 10,000
Mix A
IL-1 beta
0.35
1.52 - 3,333
Mix A
IL-2
0.79
4.57 - 10,000
Mix A
IL-4
0.54
1.52 - 3,333
Mix A
IL-5
0.11
0.51 - 1,111
Mix A
IL-6
0.12
0.51 - 1,111
Mix A
IL-8
0.19
0.51 - 1,111
Mix A
IL-9
1.32
4.57 - 10,000
Mix A
IL-10
0.96
4.57 - 10,000
Mix A
IL-12p70
0.66
4.57 - 10,000
Mix A
IL-13
2.56
4.57 - 10,000
Mix A
IL-17A
0.34
1.52 - 3,333
Mix A
MCP1
0.54
1.52 - 3,333
Mix A
MIP1a
0.08
0.51 - 1,111
Mix A
MIP1b
0.09
0.51 - 1,111
Mix A
TNF alpha
6.64
13.7 - 10,000
Mix A
Negative Control Particles
N/A
N/A
N/A -
Platform
Multiplex
Images
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Assay Summary
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Example of Human Key Cytokines (17-plex) standard curvesMean background-subtracted data values are graphed (+/- SD).