Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63) - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free
    See all Ubiquitin primary antibodies

  • Description

    Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K63-linked-Ub2-7 recombinant protein, HEK-293 and HeLa cell lysates, and Mouse and rat brain lysate. IHC-P: Human kidney carcinoma and tonsil tissue. Flow Cyt (intra): HeLa cells.

  • General notes

    ab271929 is the carrier-free version of ab179434.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)
    Flow Cytometry - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Ubiquitin (linkage-specific K63) with purified ab179434 at 1/210 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179434).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)

    Immunohistochemical staining of paraffin embedded human tonsil with purified ab179434 at a dilution of 1/500. The secondary antibody used is a HRP conjugated goat anti-rabbit (ab97051, 1/500). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179434).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)

    Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue labeling K63-linkage specific polyubiquitin with unpurified ab179434 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179434).
  • Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)
    Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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