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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I

Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)

Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

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Overview

  • Product name

    Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free
    See all Tripeptidyl peptidase II/TPPII primary antibodies
  • Description

    Rabbit monoclonal [EPR13202] to Tripeptidyl peptidase II/TPPII - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)more details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Tripeptidyl peptidase II/TPPII. The exact sequence is proprietary.
    Database link: P29144

  • General notes

    ab250179 is the carrier-free version of ab180177.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13202
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases

Images

  • Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    Western blot - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    All lanes : Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] (ab180177) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 138 kDa
    Additional bands at: 138 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab180177, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    Flow Cytometry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    This data was developed using ab180177, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of permeabilized Jurkat cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/10 (red) or a rabbit IgG (negative) (green).
  • Immunocytochemistry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    Immunocytochemistry - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    This data was developed using ab180177, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/100 (red) and DAPI staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    This data was developed using ab180177, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Tripeptidyl peptidase II/TPPII with ab180177 at 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)
    Anti-Tripeptidyl peptidase II/TPPII antibody [EPR13202] - BSA and Azide free (ab250179)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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