Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322] (ab185550)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15322] to Transferrin Receptor 2/TFR2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322]
See all Transferrin Receptor 2/TFR2 primary antibodies -
Description
Rabbit monoclonal [EPR15322] to Transferrin Receptor 2/TFR2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 and TF-1 cell lysates; Human fetal liver lysate; Human liver tissue.
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General notes
This product was previously labelled as Transferrin Receptor 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15322 -
Isotype
IgG -
Research areas
Images
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Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322] (ab185550) at 1/5000 dilution + TF-1 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 89 kDa
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Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322] (ab185550) at 1/5000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 89 kDa
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Anti-Transferrin Receptor 2/TFR2 antibody [EPR15322] (ab185550) at 1/20000 dilution + Human fetal liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 89 kDa
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Transferrin Receptor 2/TFR2 with ab185550 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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