Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPD52 antibody [EPR14219] (ab181260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14219] to TPD52
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TPD52 antibody [EPR14219]
    See all TPD52 primary antibodies

  • Description

    Rabbit monoclonal [EPR14219] to TPD52

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7, THP1 and Raji cell lysates; Human kidney and liver tissues; MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14219

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TPD52 antibody [EPR14219] (ab181260)
    Western blot - Anti-TPD52 antibody [EPR14219] (ab181260)
    All lanes : Anti-TPD52 antibody [EPR14219] (ab181260) at 1/2000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : THP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14219] (ab181260)
    Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14219] (ab181260)

    Immunocytochemistry/Immunofluorescence analysis of THP-1 cells labelling TPD52 with ab181260 at 1/1000. Cells were fixed with 100% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Western blot - Anti-TPD52 antibody [EPR14219] (ab181260)
    Western blot - Anti-TPD52 antibody [EPR14219] (ab181260)
    Anti-TPD52 antibody [EPR14219] (ab181260) at 1/5000 dilution + Raji cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (ab181260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (ab181260)

    Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling TPD52 with ab181260 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (ab181260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14219] (ab181260)

    Immunohistochemical analysis of paraffin-embedded Human Liver tissue labeling TPD52 with ab181260 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-TPD52 antibody [EPR14219] (ab181260)
    Flow Cytometry - Anti-TPD52 antibody [EPR14219] (ab181260)

    Flow cytometric analysis of MCF7 cells (2% paraformaldehyde-fixed) labeling TPD52 with ab181260 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Anti-TPD52 antibody [EPR14219] (ab181260)
    Anti-TPD52 antibody [EPR14219] (ab181260)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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