Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR060F] to BCKDHA (phospho S292)
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BCKDHA (phospho S292) antibody [BLR060F]
    See all BCKDHA primary antibodies

  • Description

    Rabbit monoclonal [BLR060F] to BCKDHA (phospho S292)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human BCKDHA (phospho S292). Using the numbering given for the precursor protein of entry NP_000700.1 (GeneID 593), the phosphorylated serine corresponds to residue 337.
    Database link: P12694

  • Positive control

    • WB: NIH/3T3, TCMK-1, HepG2, Jurkat, LNCaP, GaMG,HEK-293T, MCF-7, and differentiated 3T3-L1 whole cell lysates.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.80
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 99.81% Borate buffered saline
  • Concentration information loading...

  • Purification notes

    Recombinant antibody was purified from cell culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    BLR060F

  • Isotype

    IgG

Images

  • Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)
    Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)
    All lanes : Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : LNCaP (human prostate cancer cell line) whole cell lysate
    Lane 6 : GaMG whole cell lysate
    Lane 7 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 8 : MCF-7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 9 : Differentiated 3T3-L1 whole cell lysate

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 50 kDa



    Chemiluminescence with an exposure time of 30 seconds.

    Lower Panel: Rabbit anti-RPL10

  • Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)
    Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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