Antibodies, Proteins, Kits and Reagents for Life Science

Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR060F] to BCKDHA (phospho S292)
Suitable for: WB
Reacts with: Mouse, Human

Product name

Anti-BCKDHA (phospho S292) antibody [BLR060F]
See all BCKDHA primary antibodies
Description

Rabbit monoclonal [BLR060F] to BCKDHA (phospho S292)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human BCKDHA (phospho S292). Using the numbering given for the precursor protein of entry NP_000700.1 (GeneID 593), the phosphorylated serine corresponds to residue 337.
Database link: P12694
Positive control
- WB: NIH/3T3, TCMK-1, HepG2, Jurkat, LNCaP, GaMG,HEK-293T, MCF-7, and differentiated 3T3-L1 whole cell lysates.
General notes

This product is sold under License from Bethyl Laboratories, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.80
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99.81% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR060F
Isotype

IgG

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)

All lanes : Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : LNCaP (human prostate cancer cell line) whole cell lysate
Lane 6 : GaMG whole cell lysate
Lane 7 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 8 : MCF-7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 9 : Differentiated 3T3-L1 whole cell lysate

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 50 kDa

Chemiluminescence with an exposure time of 30 seconds.

Lower Panel: Rabbit anti-RPL10
Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961)

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