Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIM 3 antibody [BLR033F] (ab252533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR033F] to TIM 3
  • Suitable for: WB, ICC, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TIM 3 antibody [BLR033F]
    See all TIM 3 primary antibodies

  • Description

    Rabbit monoclonal [BLR033F] to TIM 3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human TIM 3 (extracellular). NP_116171.3.
    Database link: Q8TDQ0

  • Positive control

    • IHC-P: Human pleural effusion lung carcinoma and tonsil tissue. ICC: RPMI 8226 cells. WB: SR, HDLM-2, SUP-M2, KG-1 and RPMI 8226 whole cell lysates.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 99% Borate buffered saline

    Borate buffered saline
  • Concentration information loading...

  • Purity

    IgG fraction

  • Purification notes

    Purified from cell culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    BLR033F

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [BLR033F] (ab252533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [BLR033F] (ab252533)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for TIM 3 using ab252533 at 1/200 dilution in immunohistochemical analysis. HRP-conjugated goat anti-rabbit IgG secondary antibody. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [BLR033F] (ab252533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [BLR033F] (ab252533)

    Formalin-fixed, paraffin-embedded human pleural effusion lung carcinoma tissue stained for TIM 3 using ab252533 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG secondary antibody was used. DAB staining.

  • Immunocytochemistry - Anti-TIM 3 antibody [BLR033F] (ab252533)
    Immunocytochemistry - Anti-TIM 3 antibody [BLR033F] (ab252533)

    Formalin-fixed, paraffin-embedded human RPMI 8226 cells stained for TIM 3 using ab252533 at 1/200 dilution in immunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG secondary antibody. DAB staining.

  • Western blot - Anti-TIM 3 antibody [BLR033F] (ab252533)
    Western blot - Anti-TIM 3 antibody [BLR033F] (ab252533)
    All lanes : Anti-TIM 3 antibody [BLR033F] (ab252533) at 1/1000 dilution

    Lane 1 : SR whole cell lysate
    Lane 2 : MJ whole cell lysate
    Lane 3 : HDLM-2 whole cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 5 : SUP-M2 whole cell lysate
    Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 7 : KG-1 whole cell lysate
    Lane 8 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 9 : RPMI 8226 whole cell lysate
    Lane 10 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 11 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 30 seconds


    Loading control: GAPDH.

  • Anti-TIM 3 antibody [BLR033F] (ab252533)
    Anti-TIM 3 antibody [BLR033F] (ab252533)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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