Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TIM 3 antibody [BLR033F] (ab252533)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR033F] to TIM 3
Suitable for: WB, ICC, IHC-P
Reacts with: Human

Product name

Anti-TIM 3 antibody [BLR033F]
See all TIM 3 primary antibodies
Description

Rabbit monoclonal [BLR033F] to TIM 3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human TIM 3 (extracellular). NP_116171.3.
Database link: Q8TDQ0
Positive control
- IHC-P: Human pleural effusion lung carcinoma and tonsil tissue. ICC: RPMI 8226 cells. WB: SR, HDLM-2, SUP-M2, KG-1 and RPMI 8226 whole cell lysates.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline

Borate buffered saline
Concentration information loading...
Purity

IgG fraction
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR033F
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [BLR033F] (ab252533)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TIM 3 using ab252533 at 1/200 dilution in immunohistochemical analysis. HRP-conjugated goat anti-rabbit IgG secondary antibody. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 3 antibody [BLR033F] (ab252533)

Formalin-fixed, paraffin-embedded human pleural effusion lung carcinoma tissue stained for TIM 3 using ab252533 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG secondary antibody was used. DAB staining.
Immunocytochemistry - Anti-TIM 3 antibody [BLR033F] (ab252533)

Formalin-fixed, paraffin-embedded human RPMI 8226 cells stained for TIM 3 using ab252533 at 1/200 dilution in immunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG secondary antibody. DAB staining.
Western blot - Anti-TIM 3 antibody [BLR033F] (ab252533)

All lanes : Anti-TIM 3 antibody [BLR033F] (ab252533) at 1/1000 dilution

Lane 1 : SR whole cell lysate
Lane 2 : MJ whole cell lysate
Lane 3 : HDLM-2 whole cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 5 : SUP-M2 whole cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : KG-1 whole cell lysate
Lane 8 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 9 : RPMI 8226 whole cell lysate
Lane 10 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 11 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Predicted band size: 33 kDa

Exposure time: 30 seconds

Loading control: GAPDH.
Anti-TIM 3 antibody [BLR033F] (ab252533)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com