Anti-TIM 1 antibody [mAbcam75295] (ab75295)
Key features and details
- Mouse monoclonal [mAbcam75295] to TIM 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TIM 1 antibody [mAbcam75295]
See all TIM 1 primary antibodies -
Description
Mouse monoclonal [mAbcam75295] to TIM 1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TIM 1 aa 300 to the C-terminus (C terminal).
(Peptide available asab97979) -
Positive control
- This antibody gave a positive signal in the human testis and brain tissue lysates.
-
General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
-
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam75295 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-TIM 1 antibody [mAbcam75295] (ab75295) at 10 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The band at approximately 50-kDa is comparable to the molecular weight seen with other antibodies to TIM 1. TIM 1 contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.