NFAT5 overexpression 293T lysate (whole cell) (ab94270)
Overview
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Product name
NFAT5 overexpression 293T lysate (whole cell) -
General notes
ab94270 is a 293T cell transfected lysate in which Human NFAT5 has been transiently over-expressed using a pCMV-NFAT5 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes. Similarity: Contains 1 RHD (Rel-like) domain. Tissue specificity: Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Images
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SDS-PAGE - NFAT5 overexpression 293T lysate (whole cell) (ab94270)ab94270 at 15µg/lane on an SDS-PAGE gel.
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Western blot - NFAT5 overexpression 293T lysate (whole cell) (ab94270)All lanes : Anti-NFAT5 antibody - ChIP Grade (ab56997) at 1/500 dilution
Lane 1 :NFAT5 overexpression 293T lysate (whole cell) (ab94270)
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
