Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIM 4 antibody [EPR22304-3] (ab222093)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22304-3] to TIM 4
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TIM 4 antibody [EPR22304-3]
    See all TIM 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22304-3] to TIM 4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HEK-293T cells. Flow Cyt: HEK-293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22304-3

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-TIM 4 antibody [EPR22304-3] (ab222093)
    Flow Cytometry - Anti-TIM 4 antibody [EPR22304-3] (ab222093)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a myc-tagged TIM 4 expression construct labeling TIM 4 with ab222093 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue).  

    Cells were surface stained with ab222093. Then fixed with 2% PFA for 10min, followed by intracellular staining of anti-myc tag conjugated to Alexa Fluor®647.

    Gated on myc -positive population.

  • Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR22304-3] (ab222093)
    Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR22304-3] (ab222093)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TIM 4 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling TIM 4 (Green) with ab222093 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) antibody at 1/1000 dilution. Confocal image showing positive staining in HEK-293T cells transfected with a myc-tagged TIM 4 expression construct. myc-Tag (9B11) Mouse mAb (Alexa Fluor® 594 Conjugate) (ab223894) was used as the counterstain (Red) at 1/100 dilution. The nuclear counterstain was DAPI (Blue).

    Negative control: Myc only transfected HEK-293T cells.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077).

  • Anti-TIM 4 antibody [EPR22304-3] (ab222093)
    Anti-TIM 4 antibody [EPR22304-3] (ab222093)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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