Anti-TIM 1 antibody (ab47635)
Key features and details
- Rabbit polyclonal to TIM 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TIM 1 antibody
See all TIM 1 primary antibodies -
Description
Rabbit polyclonal to TIM 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A 16 amino acid peptide from near the amino terminus of human TIM 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIM 1 antibody (ab47635)All lanes : Anti-TIM 1 antibody (ab47635) at 8 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 5 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 10 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 11 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 12 : THP-1 (human monocytic leukemia cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Diluting buffer and concentration: 5% NFDM/TBST.
Primary incubated overnight at 4°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 1 antibody (ab47635)Ab47635 at 5μg/ml staining TIM1 in normal human kidney tissue section by Immunohistochemistry ( IHC-P-Formalin/PFA fixed paraffin-embedded sections).
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Western blot - Anti-TIM 1 antibody (ab47635)Anti-TIM 1 antibody (ab47635) at 8 µg/ml + NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg
Secondary
Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?Diluting buffer and concentration: 5% NFDM/TBST.
Primary incubated overnight at 4°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 1 antibody (ab47635)Ab47635 at 5μg/ml staining TIM1 in human kidney carcinoma tissue section by Immunohistochemistry (IHC-P-Formalin/PFA fixed paraffin-embedded sections).
