Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23574-405] to Thyroid Peroxidase/TPO
  • Suitable for: IHC-P, IHC-Fr, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405]
    See all Thyroid Peroxidase/TPO primary antibodies

  • Description

    Rabbit monoclonal [EPR23574-405] to Thyroid Peroxidase/TPO

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat thyroid tissue lysate. IHC-P: Mouse and rat thyroid tissue. IHC-Fr: Mouse and rat thyroid tissue.

  • General notes

    Please Note: Clone [EPR23574-405] (this product) is different to that of ab275932 [EPR23574-130] for the same target.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525) at 1/1000 dilution + Mouse thyroid tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 103 kDa
    Observed band size: 101 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

    Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Cytoplasmic staining in mouse thyroid. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/500 (1.086 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse thyroid is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    All lanes : Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525) at 1/1000 dilution

    Lane 1 : Rat thyroid tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 103 kDa
    Observed band size: 101 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    TPO is expressed in thyroid. Positive signal is observed in thyroid tissue (PMID: 29541194), no signal is observed in heart tissue.

    Exposure time: 3 minutes

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

    Immunohistochemical analysis of paraffin-embedded rat thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Cytoplasmic staining in rat thyroid. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/500 (1.086 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat thyroid is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond®  Polymer Refine Detection) was used. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    No staining in rat cardiac muscle.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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