Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23574-405] to Thyroid Peroxidase/TPO
Suitable for: IHC-P, IHC-Fr, WB
Reacts with: Mouse, Rat

Product name

Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405]
See all Thyroid Peroxidase/TPO primary antibodies
Description

Rabbit monoclonal [EPR23574-405] to Thyroid Peroxidase/TPO
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat thyroid tissue lysate. IHC-P: Mouse and rat thyroid tissue. IHC-Fr: Mouse and rat thyroid tissue.
General notes
Please Note: Clone [EPR23574-405] (this product) is different to that of ab275932 [EPR23574-130] for the same target.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23574-405
Isotype

IgG
Research areas

Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525) at 1/1000 dilution + Mouse thyroid tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 103 kDa
Observed band size: 101 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Cytoplasmic staining in mouse thyroid. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/500 (1.086 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse thyroid is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

All lanes : Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525) at 1/1000 dilution

Lane 1 : Rat thyroid tissue lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 103 kDa
Observed band size: 101 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

TPO is expressed in thyroid. Positive signal is observed in thyroid tissue (PMID: 29541194), no signal is observed in heart tissue.

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

Immunohistochemical analysis of paraffin-embedded rat thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Cytoplasmic staining in rat thyroid. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^®Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/500 (1.086 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat thyroid is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

No staining in rat cardiac muscle.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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