Recombinant human Inhibin beta A protein (Active) (ab219889)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: ELISA, SDS-PAGE
Preparation and Storage
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Images
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Functional Studies - Recombinant human Inhibin beta A protein (His tag) (ab219889)
The bio-activity of ab219889 was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically 47-66 ng/mL.
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SDS-PAGE - Recombinant human Inhibin beta A protein (His tag) (ab219889)SDS-PAGE analysis of reduced ab219889 stained overnight with Coomassie Blue.
The protein has a calculated MW of 13 kDa (mature) & 32 kDa (pro). As a result of glycosylation and Interchain disulfide bond, the protein migrates as 15 kDa (mature) and 43-48 kDa (pro) under reducing (Lane 1) condition, and 27 kDa (mature), 43-48 kDa (pro) and 60 kDa (pro & mature) under non-reducing (Lane 2) condition (SDS-PAGE).
