Anti-Thrombin antibody [BDI905] (ab20877)
![Anti-Thrombin antibody [BDI905] (ab20877)](https://www.abcam.com/ps/products/20/ab20877/Images/ab20877-30002-anti-thrombin-antibody-bdi905-immunofluorescence.jpg "Anti-Thrombin antibody [BDI905] (ab20877)")
Key features and details
- Mouse monoclonal [BDI905] to Thrombin
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Thrombin antibody [BDI905]
See all Thrombin primary antibodies -
Description
Mouse monoclonal [BDI905] to Thrombin -
Host species
Mouse -
Specificity
Ab20877 has not been tested for cross-reactivity with prothrombin therefore we do not know if it will cross react with it. -
Tested applications
Suitable for: ICC/IFmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein (purified) (Human)
Properties
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Form
Liquid -
Storage instructions
Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purification notes
The antibody has been purified from tissue culture by Ammonium sulfate precipitation, ion-exchange chromatography and gel filtration. -
Clonality
Monoclonal -
Clone number
BDI905 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Thrombin antibody [BDI905] (ab20877)ICC/IF image of ab20877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20877, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-goat mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
