Anti-MYH/MUTYH antibody (ab228722)
Key features and details
- Rabbit polyclonal to MYH/MUTYH
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MYH/MUTYH antibody
See all MYH/MUTYH primary antibodies -
Description
Rabbit polyclonal to MYH/MUTYH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human MYH/MUTYH (internal sequence). The exact sequence is proprietary.
Database link: Q9UIF7 -
Positive control
- WB: HeLa, HepG2 and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as MYH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MYH/MUTYH antibody (ab228722)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MYH/MUTYH (green) using ab228722 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Western blot - Anti-MYH/MUTYH antibody (ab228722)All lanes : Anti-MYH/MUTYH antibody (ab228722) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 60 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-MYH/MUTYH antibody (ab228722)Anti-MYH/MUTYH antibody (ab228722) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 60 kDa7.5% SDS-PAGE gel.
