Recombinant mouse IFNA4 protein (His tag) (ab276897)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse IFNA4 protein (His tag)
See all IFNA4 proteins and peptides -
Biological activity
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV).
The ED50 for this effect is typically 2-12 pg/mL. -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Mouse -
Sequence
MARLCAFLMI LVMMSYYWSA CSLGCDLPHT YNLGNKRALT VLEEMRRLPP LSCLKDRKDF GFPLEKVDNQ QIQKAQAILV LRDLTQQILN LFTSKDLSAT WNATLLDSFC NDLHQQLNDL KACVMQEPPL TQEDSLLAVR TYFHRITVYL RKKKHSLCAW EVIRAEVWRA LSSSTNLLAR LSEEKE -
Predicted molecular weight
20 kDa including tags -
Molecular weight information
The recombinant mouse IFNA4 comprises 173 amino acids and has a predicted molecular mass of 20.2 kDa. The apparent molecular mass of the protein is approximately 22-27 kDa in SDS-PAGE under reducing conditions. -
Amino acids
1 to 186 -
Tags
His tag C-Terminus -
Additional sequence information
Predicted N-terminus: Cys 25.
Specifications
Our Abpromise guarantee covers the use of ab276897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- IFN-alpha-4
- IFN-alpha4a
- INFA4
see all -
Relevance
Produced by macrophages, IFN alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Cellular localization
Secreted
Images
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Functional Studies - Recombinant Mouse IFNA4 protein (His tag) (ab276897)
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV).
The ED50 for this effect is typically 2-12 pg/mL. -
SDS-PAGE - Recombinant Mouse IFNA4 protein (His tag) (ab276897)SDS-PAGE analysis of ab276897
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276897 has not yet been referenced specifically in any publications.
Preparation and Storage
- IFN-alpha-4
- IFN-alpha4a
- INFA4
Images
-
Functional Studies - Recombinant Mouse IFNA4 protein (His tag) (ab276897)
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV).
The ED50 for this effect is typically 2-12 pg/mL. -
SDS-PAGE - Recombinant Mouse IFNA4 protein (His tag) (ab276897)SDS-PAGE analysis of ab276897