Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)
![Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)](https://www.abcam.com/ps/products/181/ab181206/Images/ab181206-287168-anti-tau-phospho-t205-antibody-epr24032-western-blot.jpg "Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2403(2)] to Tau (phospho T205)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-Tau (phospho T205) antibody [EPR2403(2)]
See all Tau primary antibodies -
Description
Rabbit monoclonal [EPR2403(2)] to Tau (phospho T205) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SK-N-BE(2) whole cell lysate and human fetal brain tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2403(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)All lanes : Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206) at 1/1000 dilution
Lane 1 : SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate
Lane 2 : SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate. The membrane was incubated with phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)](https://www.abcam.com/ps/products/181/ab181206/Images/ab181206-287166-anti-tau-phospho-t205-antibody-epr24032-dot-blot.jpg)
Dot Blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)Dot blot analysis of Tau (pS205) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS205) with ab181206 at a dilution of 1/1000. ab97051 (peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Exposure time: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST. -
![Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)](https://www.abcam.com/ps/products/181/ab181206/Images/ab181206-214756-anti-tau-phospho-t205-antibody-epr24032-western-blot.jpg)
Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)All lanes : Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206) at 1/50000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal brain treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 78 kDa
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![Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)](https://www.abcam.com/ps/products/181/ab181206/Images/ab181206-5-benefits-of-recombinant-antibodies.png)
Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206)
