Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Syk (phospho Y525) antibody [EP575(2)Y] - BSA and Azide free (ab247353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP575(2)Y] to Syk (phospho Y525) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-Syk (phospho Y525) antibody [EP575(2)Y] - BSA and Azide free
    See all Syk primary antibodies

  • Description

    Rabbit monoclonal [EP575(2)Y] to Syk (phospho Y525) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises Syk phosphorylated at Tyrosine 525.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247353 is the carrier-free version of ab62350 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247353 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y] - BSA and Azide free (ab247353)
    Dot Blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y] - BSA and Azide free (ab247353)
    This data was developed using ab62350, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBSTLane 1 sample: Syk(pY525) phospho peptide Lane 2 sample: Syk non-phospho peptideExposure time: 3 minutes
  • Western blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y] - BSA and Azide free (ab247353)
    Western blot - Anti-Syk (phospho Y525) antibody [EP575(2)Y] - BSA and Azide free (ab247353)
    All lanes : Anti-Syk (phospho Y525) antibody [EP575(2)Y] (ab62350) at 1/10000 dilution

    Lane 1 : untreated U937 cell lysates
    Lane 2 : U937 cell lysates treated with pervanadate.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti rabbit HRP at 1/2000 dilution

    Predicted band size: 72 kDa
    Observed band size: 75,80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab62350, the same antibody clone in a different buffer formulation.

    Beta tubulin shows equal lysate loading amount.

  • Anti-Syk (phospho Y525) antibody [EP575(2)Y] - BSA and Azide free (ab247353)
    Anti-Syk (phospho Y525) antibody [EP575(2)Y] - BSA and Azide free (ab247353)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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