Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Stargazin antibody [N245/36] (ab167445)

Key features and details

  • Mouse monoclonal [N245/36] to Stargazin
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Stargazin antibody [N245/36]
    See all Stargazin primary antibodies

  • Description

    Mouse monoclonal [N245/36] to Stargazin

  • Host species

    Mouse

  • Specificity

    ab167445 does not cross-react with Stargazin 3.

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Fusion protein corresponding to Rat Stargazin aa 200 to the C-terminus (C terminal).
    Database link: Q71RJ2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain tissue lysate. ICC/IF: SK-N-BE cells.

  • General notes

    The clone number has been updated from S245-36 to N245/36, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    N245/36

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Stargazin antibody [N245/36] (ab167445)
    Immunocytochemistry/ Immunofluorescence - Anti-Stargazin antibody [N245/36] (ab167445)

    4% formaldehyde-fixed SK-N-BE cells labeling Stargazin using ab167445 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

  • Western blot - Anti-Stargazin antibody [N245/36] (ab167445)
    Western blot - Anti-Stargazin antibody [N245/36] (ab167445)
    Lane 1 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/100 dilution
    Lane 2 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/250 dilution
    Lane 3 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/500 dilution
    Lane 4 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/1000 dilution

    All lanes : Rat brain lysate

    Predicted band size: 36 kDa

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