Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17847-32] to STARD4 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free
    See all STARD4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17847-32] to STARD4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251360 is the carrier-free version of ab202060. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251360 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunocytochemistry - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    Immunocytochemistry - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)

    This data was developed using ab202060, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling STRAD4 with ab202060 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:
    -ve control 1: ab202060 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    All lanes : Anti-STARD4 antibody [EPR17847-32] (ab202060) at 1/1000 dilution

    Lane 1 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 4 : U937 (Human histiocytic lymphoma cells) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 3 minutes


    This data was developed using ab202060, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    All lanes : Anti-STARD4 antibody [EPR17847-32] (ab202060) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 3 minutes


    This data was developed using ab202060, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    All lanes : Anti-STARD4 antibody [EPR17847-32] (ab202060) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : Rat liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 3 minutes


    This data was developed using ab202060, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    All lanes : Anti-STARD4 antibody [EPR17847-32] (ab202060) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
    Lane 5 : Mouse liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 1 minute


    This data was developed using ab202060, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    Immunoprecipitation - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    This data was developed using ab202060, the same antibody clone in a different buffer formulation.STARD4 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate with ab202060 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202060 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: NIH/3T3 whole cell lysate 10 µg (Input). Lane 2: ab202060 IP in NIH/3T3 whole cell lysate Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202060 in NIH/3T3 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds.
  • Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)
    Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (ab251360)

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