Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16466] to SNAPC1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free
    See all SNAPC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16466] to SNAPC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251226 is the carrier-free version of ab197015.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    Western blot - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    All lanes : Anti-SNAPC1 antibody [EPR16466] (ab197015) at 1/5000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    This data was developed using ab197015, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    Western blot - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    Anti-SNAPC1 antibody [EPR16466] (ab197015) at 1/5000 dilution + Human fetal brain tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    This data was developed using ab197015, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    Immunocytochemistry - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    This data was developed using ab197015, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling SNAPC1 with ab197015 at 1/250 dilution, followed by Alexa Fluor®488 Goat Anti-Rabbit IgG H&L (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab197015 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Flow Cytometry - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    Flow Cytometry - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    This data was developed using ab197015, the same antibody clone in a different buffer formulation.Flow cytometric analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling SNAPC1 with ab197015 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)
    Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (ab251226)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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