Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)
![Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)](https://www.abcam.com/ps/products/239/ab239855/Images/ab239855-395416-anti-smurf-2-antibody-ep629y3-western-blot-human-knockout-lysate.jpg "Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP629Y3] to SMURF 2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free
See all SMURF 2 primary antibodies -
Description
Rabbit monoclonal [EP629Y3] to SMURF 2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, SH-SY5Y and U-2 OS cell lysates.
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General notes
Ab239855 is the carrier-free version of ab53316. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab239855 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP629Y3 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)All lanes : Anti-SMURF 2 antibody [EP629Y3] (ab53316) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SMURF2 knockout HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab53316).
Lanes 1-3: Merged signal (red and green). Green - ab53316 observed at 86 kDa. Red - loading control ab8245 observed at 36 kDa.
ab53316 Anti-SMURF 2 antibody [EP629Y3] was shown to specifically react with SMURF 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265231 (knockout cell lysate ab257691) was used. Wild-type and SMURF 2 knockout samples were subjected to SDS-PAGE. ab53316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)](https://www.abcam.com/ps/products/239/ab239855/Images/ab239855-393751-anti-smurf-2-antibody-ep629y3-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)All lanes : Anti-SMURF 2 antibody [EP629Y3] (ab53316) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SMURF2 knockout HeLa cell lysate
Lane 3 : U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab53316).
Lanes 1-3: Merged signal (red and green). Green - ab53316 observed at 86 kDa. Red - loading control ab8245 observed at 36 kDa.
ab53316 Anti-SMURF 2 antibody [EP629Y3] was shown to specifically react with SMURF 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264806 (knockout cell lysate ab257690) was used. Wild-type and SMURF 2 knockout samples were subjected to SDS-PAGE. ab53316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)](https://www.abcam.com/ps/products/239/ab239855/Images/ab239855-3-benefits-of-recombinant-antibodies.png)
Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)
