Anti-SMURF 2 antibody [EP629Y3] (ab53316)
![Anti-SMURF 2 antibody [EP629Y3] (ab53316)](https://www.abcam.com/ps/products/53/ab53316/Images/ab53316-395417-anti-smurf-2-antibody-ep629y3-western-blot-human-knockout-lysate.jpg "Anti-SMURF 2 antibody [EP629Y3] (ab53316)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP629Y3] to SMURF 2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SMURF 2 antibody [EP629Y3]
See all SMURF 2 primary antibodies -
Description
Rabbit monoclonal [EP629Y3] to SMURF 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human SMURF 2 aa 200-300. The exact sequence is proprietary.
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Positive control
- WB: HeLa, U-2 OS and SH-SY-5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP629Y3 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMURF 2 antibody [EP629Y3] (ab53316)All lanes : Anti-SMURF 2 antibody [EP629Y3] (ab53316) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SMURF2 knockout HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaLanes 1-3: Merged signal (red and green). Green - ab53316 observed at 86 kDa. Red - loading control ab8245 observed at 36 kDa.
ab53316 Anti-SMURF 2 antibody [EP629Y3] was shown to specifically react with SMURF 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265231 (knockout cell lysate ab257691) was used. Wild-type and SMURF 2 knockout samples were subjected to SDS-PAGE. ab53316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SMURF 2 antibody [EP629Y3] (ab53316)](https://www.abcam.com/ps/products/53/ab53316/Images/ab53316-393638-anti-smurf-2-antibody-ep629y3-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-SMURF 2 antibody [EP629Y3] (ab53316)All lanes : Anti-SMURF 2 antibody [EP629Y3] (ab53316) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SMURF2 knockout HeLa cell lysate
Lane 3 : U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaLanes 1-3: Merged signal (red and green). Green - ab53316 observed at 86 kDa. Red - loading control ab8245 observed at 36 kDa.
ab53316 Anti-SMURF 2 antibody [EP629Y3] was shown to specifically react with SMURF 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264806 (knockout cell lysate ab257690) was used. Wild-type and SMURF 2 knockout samples were subjected to SDS-PAGE. ab53316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SMURF 2 antibody [EP629Y3] (ab53316)](https://www.abcam.com/ps/products/53/ab53316/Images/ab53316-66099-anti-smurf-2-antibody-ep629y3-western-blot.png)
Western blot - Anti-SMURF 2 antibody [EP629Y3] (ab53316)Anti-SMURF 2 antibody [EP629Y3] (ab53316) at 1/500 dilution + SH-SY-5Y cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa
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![Anti-SMURF 2 antibody [EP629Y3] (ab53316)](https://www.abcam.com/ps/products/53/ab53316/Images/ab53316-4-benefits-of-recombinant-antibodies.png)
Anti-SMURF 2 antibody [EP629Y3] (ab53316)
