Human Nrf2 ELISA Kit (ab277397)
Key features and details
- Sensitivity: 14 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Nrf2 ELISA Kit
See all Nrf2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14 pg/ml -
Range
13.72 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105.3 69% - 144% Plasma 85.07 68% - 102% Cell culture media 98.76 86% - 106% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Nrf2 ELISA Kit (ab277397) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Nrf2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Nrf2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Nrf2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Nrf2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Nrf2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human Nrf2 Antibody 2 vials Anti-Human Nrf2 coated Microplate 1 unit Human Nrf2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. -
Tissue specificity
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. -
Sequence similarities
Belongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain. -
Domain
Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus. -
Post-translational
modificationsPhosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. - Information by UniProt
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Alternative names
- erythroid derived 2
- HEBP1
- like 2
see all -
Database links
- Entrez Gene: 4780 Human
- Omim: 600492 Human
- SwissProt: Q16236 Human
- Unigene: 744006 Human
Images
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Human Nrf2 ELISA kit (ab277397) Standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.