Mouse CD40 Matched Antibody Pair Kit (ab213469)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 15.6 pg/ml - 1000 pg/ml
Overview
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Product name
Mouse CD40 Matched Antibody Pair Kit
See all CD40 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.4 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CD40 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CD40.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab206307. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse CD40 Capture Antibody 2 x 50µg 1 x 50µg Mouse CD40 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse CD40 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- AI326936
- B cell associated molecule CD40
- B cell surface antigen CD40
see all -
Database links
- Entrez Gene: 21939 Mouse
- SwissProt: P27512 Mouse
- Unigene: 271833 Mouse
Images
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Mouse CD40 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse CD40 Matched Antibody Pair Kit (ab213469)To learn more about the advantages of recombinant antibodies see here.
