Anti-SMC3 (phospho S1083) antibody (ab264351)
Key features and details
- Rabbit polyclonal to SMC3 (phospho S1083)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMC3 (phospho S1083) antibody
See all SMC3 primary antibodies -
Description
Rabbit polyclonal to SMC3 (phospho S1083) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
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Immunogen
Synthetic peptide within Human SMC3 (phospho S1083). The exact sequence is proprietary. NP_005436.1
Database link: Q9UQE7 -
Positive control
- WB: HeLa (treated with ectoposide) whole cell lysate. IP: HeLa (treated with ectoposide) whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC3 (phospho S1083) antibody (ab264351)All lanes : Anti-SMC3 (phospho S1083) antibody (ab264351) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) (treated with 100 uM ectoposide for 4 hours) whole cell lysate
Lane 2 : HeLa (treated with 100 uM ectoposide + 100 uM phosphatase for 4 hours) whole cell lysate
Lane 3 : Untreated HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 141 kDaExposure times.
Upper panel: 3 minutes.
Lower panel: 10 seconds.
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Immunoprecipitation - Anti-SMC3 (phospho S1083) antibody (ab264351)SMC3 (phospho S1083) was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264351 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264351 at 1 μg/ml.
Lane 1: ab264351 IP in HeLa (100 uM ectoposide for 4 hours) whole cell lysate.
Lane 2: ab264351 IP in HeLa (untreated) whole cell lysate.
Lane 3: Control IgG IP in Hela (ectoposide for 4 hours) whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
