Anti-SLC12A6/KCC3 antibody (ab92951)
Key features and details
- Rabbit polyclonal to SLC12A6/KCC3
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SLC12A6/KCC3 antibody
See all SLC12A6/KCC3 primary antibodies -
Description
Rabbit polyclonal to SLC12A6/KCC3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Kidney; Skeletal Muscle.
-
General notes
This product was previously labelled as SLC12A6
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).Target
-
Function
Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells. -
Tissue specificity
Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level). -
Involvement in disease
Agenesis of the corpus callosum, with peripheral neuropathy -
Sequence similarities
Belongs to the SLC12A transporter family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Basolateral cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 9990 Human
- Omim: 604878 Human
- SwissProt: Q9UHW9 Human
- Unigene: 510939 Human
-
Alternative names
- ACCPN antibody
- Electroneutral potassium-chloride cotransporter 3 antibody
- Furosemide sensitive KCl cotransporter 3 antibody
see all
Images
-
Western blot - Anti-SLC12A6/KCC3 antibody (ab92951)All lanes : Anti-SLC12A6/KCC3 antibody (ab92951) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 122,128 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesBlocked with 5% Milk for 1 hour. This antibody should recognise both isoforms of SLC12A6/KCC3. It is possible that we are detecting isoform 1 (128-kDa) in human kidney tissue lysate and isoform 2 (122-kDa) in human skeletal muscle lysate.
-
Immunocytochemistry/ Immunofluorescence - Anti-SLC12A6/KCC3 antibody (ab92951)ICC/IF image of ab92951 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92951 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa cells at 1ug/ml, and in Methanol fixed (100%, 5min) HepG2 cells at 1ug/ml
Protocols
Datasheets and documents
References (1)
ab92951 has been referenced in 1 publication.
- Loureiro CA et al. Tyrosine phosphorylation modulates cell surface expression of chloride cotransporters NKCC2 and KCC3. Arch Biochem Biophys 669:61-70 (2019). PubMed: 31145900
Images
-
Western blot - Anti-SLC12A6/KCC3 antibody (ab92951)All lanes : Anti-SLC12A6/KCC3 antibody (ab92951) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 122,128 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesBlocked with 5% Milk for 1 hour. This antibody should recognise both isoforms of SLC12A6/KCC3. It is possible that we are detecting isoform 1 (128-kDa) in human kidney tissue lysate and isoform 2 (122-kDa) in human skeletal muscle lysate.
-
Immunocytochemistry/ Immunofluorescence - Anti-SLC12A6/KCC3 antibody (ab92951)
ICC/IF image of ab92951 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92951 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa cells at 1ug/ml, and in Methanol fixed (100%, 5min) HepG2 cells at 1ug/ml
