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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-SHP1 antibody (ab60268)

Anti-SHP1 antibody (ab60268)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to SHP1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SHP1 antibody
    See all SHP1 primary antibodies
  • Description

    Goat polyclonal to SHP1
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both reported isoforms (NP_536858.1and NP_002822.2). Please note this antibody was designed using the mouse sequence, which differs by 1 amino acid from the human sequence.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    C-KASRTSSKHKEE

    , corresponding to internal sequence amino acids 551-562 of Mouse SHP1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human Liver lysates, Mouse and Rat thymus tissue lysates; IHC-P: Human Thyroid tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development

Images

  • Western blot - Anti-SHP1 antibody (ab60268)
    Western blot - Anti-SHP1 antibody (ab60268)
    Anti-SHP1 antibody (ab60268) at 0.1 µg/ml + Human Liver lysate (35µg protein in RIPA buffer)

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody (ab60268)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody (ab60268)

    ab60268 (5 µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6.

  • Western blot - Anti-SHP1 antibody (ab60268)
    Western blot - Anti-SHP1 antibody (ab60268)
    Lane 1 : Anti-SHP1 antibody (ab60268) at 0.01 µg/ml
    Lane 2 : Anti-SHP1 antibody (ab60268) at 0.1 µg/ml

    Lane 1 : Mouse Thymus lysate (35µg protein in RIPA buffer)
    Lane 2 : Rat Thymus lysate (35µg protein in RIPA buffer)

    Predicted band size: 68 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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