Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16682] to Serine racemase - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Serine racemase antibody [EPR16682] - BSA and Azide free
    See all Serine racemase primary antibodies

  • Description

    Rabbit monoclonal [EPR16682] to Serine racemase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251323 is the carrier-free version of ab200833. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251323 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)
    Western blot - Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)
    All lanes : Anti-Serine racemase antibody [EPR16682] (ab200833) at 1/5000 dilution

    Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
    Lane 2 : Human fetal brain tissue lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : HEK-293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    This data was developed using ab200833, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)
    Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)

    This data was developed using ab200833, the same antibody clone in a different buffer formulation.

    Serine racemase was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK293 whole cell lysate 10 µg (Input).

    Lane 2: ab200833 IP in HEK293 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in HEK293 whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)
    Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)
    This data was developed using ab200833, the same antibody clone in a different buffer formulation.Serine racemase was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: LNCaP whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in LNCaP whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in LNCaP whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds
  • Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)
    Anti-Serine racemase antibody [EPR16682] - BSA and Azide free (ab251323)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.