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Anti-SDHAF1 antibody [EPR13380] (ab185222)

Price and availability

264 681 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-SDHAF1 antibody [EPR13380] (ab185222)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13380] to SDHAF1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-SDHAF1 antibody [EPR13380]
    See all SDHAF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13380] to SDHAF1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, MCF7 and 293 cell lysates; 293 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.01% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13380
  • Isotype

    IgG

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] (ab185222)
    Immunocytochemistry/ Immunofluorescence - Anti-SDHAF1 antibody [EPR13380] (ab185222)

    Immunofluorescent analysis of 293 cells (4% paraformaldehyde-fixed) labeling SDHAF1 with ab185222 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and Counterstained with DAPI (blue).

  • Western blot - Anti-SDHAF1 antibody [EPR13380] (ab185222)
    Western blot - Anti-SDHAF1 antibody [EPR13380] (ab185222)
    All lanes : Anti-SDHAF1 antibody [EPR13380] (ab185222) at 1/1000 dilution

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : MCF7 mitochondria fraction lysate
    Lane 3 : MCF7 mitochondria control lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-SDHAF1 antibody [EPR13380] (ab185222)
    Anti-SDHAF1 antibody [EPR13380] (ab185222)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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