Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [MNB1] to Pentraxin 3/PTX3 - BSA and Azide free
  • Suitable for: WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free
    See all Pentraxin 3/PTX3 primary antibodies

  • Description

    Rat monoclonal [MNB1] to Pentraxin 3/PTX3 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, IHC-Frmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC, 3T3-L1 and MDA-MB-231 whole cell lysate. LPS, Brefeldin treated HUVEC whole cell lysate. Mouse and rat placenta tissue lysate. IHC-Fr: Mouse and rat placenta tissue.

  • General notes

    ab264487 is the carrier-free version of ab90806. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab264487 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)
    Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)
    All lanes : Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806) at 1/1000 dilution

    Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 2 : HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 41 kDa


    Exposure time: 15 seconds


    This data was developed using ab90806, the same antibody clone in a different buffer formulation.

    The expression profile observed is consistent with what has been described in the literature (PMID:17389238).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)
    Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)

    This data was developed using ab90806, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse placenta tissue labeling Pentraxin 3/PTX3 with ab90806 at 1/20 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on mouse placenta was observed. Nuclear counterstain is DAPI (Blue).
    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)
    Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)
    All lanes : Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806) at 1/1000 dilution

    Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Mouse placenta tissue lysate
    Lane 3 : Rat placenta tissue lysate
    Lane 4 : 3T3-L1 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 41 kDa



    This data was developed using ab90806, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2: 15 seconds; Lane 3: 3 seconds; Lane 4: 180 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)
    Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)

    This data was developed using ab90806, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen rat placenta tissue labeling Pentraxin 3/PTX3 with ab90806 at 1/20 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on rat placenta was observed. Nuclear counterstain is DAPI (Blue).
    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)
    Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)

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