Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15341-56] to UCHL5IP
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-UCHL5IP antibody [EPR15341-56]
    See all UCHL5IP primary antibodies

  • Description

    Rabbit monoclonal [EPR15341-56] to UCHL5IP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat and RAW 264.7 cell lysates; Human endometrium adenocarcinoma, Human liver and Mouse kidney tissues; Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15341-56

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    All lanes : Anti-UCHL5IP antibody [EPR15341-56] (ab192616) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Western blot - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Anti-UCHL5IP antibody [EPR15341-56] (ab192616) at 1/1000 dilution + RAW 264.7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 41 kDa

  • Immunoprecipitation - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Immunoprecipitation - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab192616 at 1/150 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Flow Cytometry - Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

    Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling UCHL5IP with ab192616 at 1/480 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-UCHL5IP antibody [EPR15341-56] (ab192616)
    Anti-UCHL5IP antibody [EPR15341-56] (ab192616)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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