Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C7orf42 antibody [EPR15381] (ab185948)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15381] to C7orf42
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-C7orf42 antibody [EPR15381]
    See all C7orf42 primary antibodies

  • Description

    Rabbit monoclonal [EPR15381] to C7orf42

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela, MCF-7, A549, HepG2, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates. Hela cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15381

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
    Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
    All lanes : Anti-C7orf42 antibody [EPR15381] (ab185948) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa



    Blocking buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-C7orf42 antibody [EPR15381] (ab185948)
    Flow Cytometry - Anti-C7orf42 antibody [EPR15381] (ab185948)

    Flow cytometry analysis of HeLa cells usingab185948 at 1/210 dilution.  Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution.  Fixative: 2% paraformaldehyde.  Isotype control: Rabbit monoclonal IgG.

  • Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
    Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
    All lanes : Anti-C7orf42 antibody [EPR15381] (ab185948) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa



    Blocking buffer: 5% NFDM/TBST

  • Anti-C7orf42 antibody [EPR15381] (ab185948)
    Anti-C7orf42 antibody [EPR15381] (ab185948)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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