Human Nectin-4 ELISA Kit (ab264607)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 98.62 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Nectin-4 ELISA Kit
See all Nectin-4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4.5% Inter-assay Sample n Mean SD CV% Extract 3 6.6% -
Sample type
Cell culture supernatant, Urine, Serum, Cell Lysate, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
98.62 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 93 89% - 99% Urine 89 85% - 98% Serum 89 82% - 98% Cell Lysate 112 105% - 121% Hep Plasma 87 82% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Nectin-4 ELISA Kit (ab264607) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nectin-4 protein in cell lysate, hep plasma, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Nectin-4 with 98.62 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Nectin-4 is a cell adhesion molecule located in the epithelial adherens junctions. Mutations in Nectin-4 lead to Ectodermal dysplasia-syndactyly syndrome, a condition where there is abnormal development of two or more ectodermal structures (like hair, tooth and nails). A fragment of Nectin-4 is shed and circulates in serum in certain types of cancers. Additionally, Nectin-4 is also a receptor for the measles virus.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Nectin-4 Capture Antibody 1 x 600µl 10X Human Nectin-4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Nectin-4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. -
Tissue specificity
Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. -
Involvement in disease
Defects in PVRL4 are the cause of ectodermal dysplasia-syndactyly syndrome type 1 (EDSS1) [MIM:613573]. EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsThe soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE. -
Cellular localization
Cell membrane. Cell junction > adherens junction. Colocalizes with MLLT4 at cadherin-based adherens junctions and Secreted. The secreted form is found in breast tumor patients. - Information by UniProt
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Alternative names
- DKFZp686K05193
- EDSS1
- Ig superfamily receptor LNIR
see all -
Database links
- Entrez Gene: 81607 Human
- Omim: 609607 Human
- SwissProt: Q96NY8 Human
- Unigene: 492490 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Nectin-4 standard curve in Sample Diluent 25BS + Enhancer.The Nectin-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Nectin-4 in human pooled sex urine samples.The concentrations of Nectin-4 were measured in duplicates, interpolated from the Nectin-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nectin-4 concentration was determined to be 7.34 ng/mL in urine.
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Interpolated concentrations of native Nectin-4 in human MCF7 based on a 62.50 µg/mL extract load.The concentrations of Nectin-4 were measured in duplicate and interpolated from the Nectin-4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nectin-4 concentration was determined to be 1.44 ng/mL in MCF7 cell culture extract.
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Sandwich ELISA - Human Nectin-4 ELISA Kit (ab264607)To learn more about the advantages of recombinant antibodies see here.
