Anti-Staphylococcus aureus antibody (ab20920)
Key features and details
- Rabbit polyclonal to Staphylococcus aureus
- Suitable for: ICC/IF, IHC-Fr
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Overview
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Product name
Anti-Staphylococcus aureus antibody
See all Staphylococcus aureus primary antibodies -
Description
Rabbit polyclonal to Staphylococcus aureus -
Host species
Rabbit -
Specificity
Soluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms. -
Tested applications
Suitable for: ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Staphylococcus aureus strain ATCC 27660 (mixture of intact organisms and lysed cells).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Staphylococcus aureus antibody (ab20920) This image is courtesy of an Abreview submitted by Dr Dagmar Salber
ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice. Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C. An Alexa Fluor® 568 goat anti-rabbit was used as the secondary
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Immunocytochemistry/ Immunofluorescence - Anti-Staphylococcus aureus antibody (ab20920) Image courtesy of an anonymous Abreview.ab20920 staining Staphylococcus aureus in human keratinocytes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in methanol, permeabilized using 0.1% saponin/ PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab20920 at 10µg/ml for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.
Red: cadherinBlue: DAPI
Green: anti-Staphylococcus aureus (ab20920)