Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651)
Key features and details
- Rabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase
- Suitable for: ICC/IF, ICC, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Trypanosoma brucei phosphoglycerate kinase antibody -
Description
Rabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ICC, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide derived from the C terminal domain of Trypanosoma brucei phosphoglycerate kinase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651) This image is courtesy of an Abreview submitted by Susan RouseAll lanes : Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651) at 1/200 dilution (in TBS + 5% milk for 12 hours at 4°C)
Lane 1 : Whole cell lysate of Trypanosoma brucei - procyclic form
Lane 2 : Whole cell lysate of Trypanosoma brucei - bloodstream
Lysates/proteins at 20000000 cells per lane.
Secondary
All lanes : A biotin-conjugated Goat anti-rabbit IgG polyclonal at 1/200 dilution
Predicted band size: 55 kDa
Observed band size: 65,75 kDa why is the actual band size different from the predicted?
Blocking Step:5% Milk for 1 hour at 25°C
Detection Method: DAB