Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free (ab251116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16230] to NDUFB10 - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free
    See all NDUFB10 primary antibodies

  • Description

    Rabbit monoclonal [EPR16230] to NDUFB10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251116 is the carrier-free version of ab192245. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251116 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free (ab251116)
    Western blot - Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free (ab251116)
    All lanes : Anti-NDUFB10 antibody [EPR16230] (ab192245) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    This data was developed using ab192245, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free (ab251116)
    Immunocytochemistry - Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free (ab251116)
    This data was developed using ab192245, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB10 with ab192245 at 1/50 dilution ( 6 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).The two negative controls are ab192245 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
  • Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free (ab251116)
    Anti-NDUFB10 antibody [EPR16230] - BSA and Azide free (ab251116)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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